Fig. 6. Effect of thrombin on HUVEC-dependent plasmin generation. Following mock () or 8 nM thrombin (•) treatment, HUVECs were incubated with 5 nM recombinant tPA for 20 minutes at 37°C. Lys-plasminogen (A) or Glu-plasminogen (B) were then added at a concentration of 150 nM, aliquots were removed at the indicated times and assayed for plasmin activity by chromogenic substrate (S2251) reactivity (n=3, ±s.d.).