Fig. 1. Leptomycin B (LMB) affects the intracellular localization of rpS5-EGFP and rpL29-EGFP. (A) Localization of rpS5-EGFP and rpL29-EGFP at different times after transfection. HeLa cells were grown on coverslips and transiently transfected with constructs coding for EGFP fusions of rpS5 or rpL29. Cells were fixed with 3.7% paraformaldehyde and further processed for analysis by fluorescence microscopy at 16 hours, 25 hours and 38 hours after transfection, respectively. (B) LMB-sensitive localization of rpS5-EGFP and rpL29-EGFP. HeLa cells were transiently transfected with constructs coding for EGFP fusions of rpS5 and rpL29, respectively. After 16 hours, cells were transferred into fresh medium with or without LMB (10 ng/ml). Five or 18 hours later, cells were fixed and analyzed by fluorescence microscopy.