Fig. 1. (A) DD-PCR of cDNA preparations from control (C) and PTHrP-treated (T) 8701-BC cells. The arrow points to a band of approximately 180 bp, selectively displayed in lane T (detail of a 6% sequencing PAGel, silver staining). (B) PCR amplification of the 168 bp product of hsbp1 cDNA in control (C) and PTHrP-treated cells (T). A band of the expected size can be observed in both lanes. Agarose gel 2%, ethidium bromide stain. M=100 bp size marker. (C) SM-PCR for hsbp1. Representative plot of normalized data versus cycle numbers fit with an exponential curve for control (s) and PTHrP-treated (•) 8701-BC cells.