Fig. 3. (Top) Western immunodetection of hsf1, hsf2 and hsp90 in lysates of control (A) and PTHrP-treated (B-E) 8701-BC cells grown in unsupplemented medium (B) or supplemented with 600 nM hsbp1-asODN (C), 1 µg geldanamycin/ml (D) or 100 µM quercetin (E). (Bottom) Coomassie Blue stain of SDS-PAGel after protein transfer to check loading.