Fig. 3. Frequencies of mildly spread nuclei that display a single centromere FISH signal (cen cluster) at the indicated time points after induction of meiosis in a diploid wild-type strain (2n WT), a tetraploid (4n), a sir3 diploid (2n sir3D) and a sir3 spo13 haploid (1n sir3D) SK1 strain. In the diploid wild-type and the 1n sir3D strain, centromere clustering is resolved at a similar pace. In tetraploid meiosis, cen clustering resolves only gradually, as is the case for the 2n sir3D strain after 180 minutes in sporulation medium (SPM; compare Fig. 5).