Fig. 4. Relative frequencies of nuclei with a single telomere FISH cluster (bouquet) obtained in limited nuclear spreads of the following SK1 strains. Diploid wild-type (2n WT; data taken from Trelles-Sticken et al., 1999), tetraploid (4n) and sir3 diploid (2n sir3D) and haploid sir3 spo13 (1n sir3D). The data shown are corrected for the initial frequencies in that the t=0 values (6% in diploid WT, 2% in 2n sir3D, 0% in tetraploid and 2% in haploid) were subtracted from all values of the subsequent time points. Initial increase in bouquet frequencies is similar in diploid WT, haploid and diploid sir3 meioses, whereas tetraploid meiosis displays a substantial delay in bouquet occurrence. In sir3, haploid and diploid meiosis a further increase in bouquet frequency pauses after 180 minutes Two peaks in the mutant and tetraploid time course suggest that physiological sub-populations progress through meiosis consecutively.