(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Centromere and telomere analysis of Ndj1-HA-expressing haploid control and kar3{Delta} SK1 strains after transfer to sporulation medium (SPM). The frequencies (based on >100 nuclei per time point) of nuclei with one centromere FISH cluster in meiotic time courses of haploid sir3{Delta} spo13{Delta} control [cen cluster (1n control)] and haploid kar3{Delta} sir3{Delta} spo13{Delta} [cen cluster (1n kar3{Delta})] drop at similar rates after the induction of meiosis. In the 1n kar3{Delta} time course, cen-cluster dissolution stalled between 180 and 240 minutes. All values were normalized with the values at 0 minutes (58% in WT; 56% in kar3{Delta}) set to 100%. The frequency of bouquet nuclei with one peripheral Ndj1-HA IF telomere cluster peaks at 300 minutes in the haploid control [Ndj1-HA bouquets (1n control)] and kar3{Delta} [Ndj1-HA bouquets (1n kar3{Delta})] SK1 strain. Ndj1-HA bouquet nuclei are more abundant at all time points in kar3{Delta} meiosis. The bouquet frequencies shown are given as the percentage of haploid Ndj1-HA-expressing (meiotic) cells (74% in the WT; 48% in kar3{Delta} mutant; Fig. 8). It appears that bouquet nuclei accumulate in absence of Kar3p.