Fig. 2. Subcellular distribution of WIP in the presence or absence of PDGF. Fluorescence micrographs of NIH 3T3 fibroblasts transfected with control plasmid (3T3pcDNA) (A) or with plasmid containing WIP coding sequence (3T3pcDNA-WIP) (B). Cells were grown and fixed as described in Fig. 1A and then labeled with rabbit serum specific for WIP followed by anti-rabbit Alexa488 (anti-WIP) plus TRITC-phalloidin to visualize actin filaments (Actin). Arrows point to dorsal/circular ruffles areas. Magnification 600x.