Fig. 3. Distribution of WIP along the membrane ruffle structure by confocal microscopy. NIH 3T3pcDNA-WIP fibroblasts were grown, stimulated with PDGF, and stained as described in Fig. 2. Immunocytochemical analysis was carried out using a polyclonal antibody against a WIP peptide (anti-WIP) and F-actin was stained with TRITC-phalloidin (Actin). 11 equidistant stacks were recorded with a confocal microscope system. The bottom, middle and top stacks of a representative field of cells containing ruffles (arrows) are shown. Magnification 1000x.