Fig. 5. PDGF-induced actin reorganization is altered in WIP^-/- primary fibroblasts. (A) Lysates of lung-derived fibroblasts (Fib) from WIP^+/+ and WIP^-/- mice were subjected to western blot analysis using anti-WIP rabbit serum as probe. (B) WIP^+/+ and WIP^-/- primary murine fibroblasts were serum starved (Medium) or serum starved and stimulated with PDGF for different times (PDGF). TRITC-phalloidin-stained cells were visualized on a Nikon Eclipse E800 microscope using 40x or 20x objectives. Lower magnification fields are depicted at 15 minutes to include more cells, which represent a more accurate proportion of ruffling cells. Arrows indicate ruffle formation.