Fig. 2. Stabilized Cyclin A does not inhibit PIM-myc degradation. Stabilized Cyclin A was expressed in alternating segments using prd-GAL4 and UAS-CycA1–170 (which results in a more extensive metaphase delay than UAS-CycA1-53, shown in Fig. 1). In addition, the embryos expressed PIM-myc under control of the pim regulatory region throughout the epidermis. Epidermal regions with expression of stabilized Cyclin A on the right but not on the left side of the dashed line are shown at high magnification after labeling with a DNA stain (A, B; DNA, red in B), anti-Cyclin B (B,C; CYCB, green in B) and anti-myc (D, PIM-myc). Arrested metaphase cells in regions with stabilized Cyclin A were found to lack PIM-myc (see arrowheads).