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Fig. 2. Confocal microscopy of p65-dsRed translocation. Green fluorescence represents either EGFP-GR (A) or EGFP (B); red fluorescence represents p65-dsRed. Times after TNF{alpha} treatment (minutes) are shown. (A) Merged images of p65-dsRed and EGFP-GR. p65-dsRed nuclear localization is very brief in cells expressing EGFP-GR and treated with 10 nM dexamethasone for 40 minutes prior to TNF{alpha} (10 ng ml-1) treatment. (B) p65-dsRed and EGFP fluorescence are shown as separate channels for clarification. p65-dsRed (left) shows increased nuclear localization in cells expressing control EGFP (right) and treated with TNF{alpha} (10 ng ml-1).