(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Repression of p65 activation by IL-4/STAT6. HeLa cells transiently transfected with p65-dsRed and either EGFP or EGFP-STAT6 were pretreated with 10 ng ml-1 IL-4 or medium for 40 minutes. Subsequent stimulation with 10 ng ml-1 TNF{alpha} was followed by confocal microscopy for 80 minutes. (A) Fluorescence images of cells expressing p65-dsRed (left; red fluorescence) and EGFP-STAT6 (right; green fluorescence) treated with IL-4 and TNF{alpha}, showing marked inhibition of p65-dsRed translocation compared with cells expressing EGFP treated with TNF{alpha} (Fig. 2B). Positions of nuclei are highlighted in grey in the first EGFP-STAT6 image. Times after TNF{alpha} treatment are shown in minutes. (B) Quantification of p65-dsRed translocation inhibition by activation and expression of EGFP-STAT6. p65-dsRed localization was analysed and quantified as described in Fig. 3. Each treatment was performed in triplicate with a minimum of 16 cells per experiment. Data plotted represent mean nuclear:cytoplasmic ratio per cell±s.d.(n–1) for each experiment.