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Fig. 2. Characterization of ZLAP2-specific antibodies and proteins encoded by ZLAP2 cDNAs. (A) Total proteins from zebrafish testicle (lane 1), liver (lane 2), blastula (lane 3) and ovary (lane 4) were separated by SDS-PAGE (11% acrylamide) and immunoblotted with the ZLAP2-serum1. A typical radiogram is shown. (B) Coupled in vitro transcription and translation of the cDNAs coding for ZLAP2{gamma} (lane 1), ZLAP2ß (lane 2) and ZLAP2{omega} (lane 3). [35S]-Methionine-labeled polypetides were separated by SDS-PAGE (11% acrylamide) and visualized by fluorography. The additional smaller polypeptides (B, lane 3) with mobilities of ZLAP2{gamma} and ZLAP2ß probably result from the internal start of translation at triplets coding for methionine at positions 241 and 447/448 of ZLAP2{omega}. Molecular masses of reference proteins (in kDa) are marked in (A).