Fig. 7. The behaviour of LAP2 during mitosis in zebrafish AB9 cells. Immunofluorescence microscopy of an AB9 cell at metaphase (A; A', phase contrast) and anaphase (B; B', phase contrast), after staining with ZLAP2-specific antibodies. Note that metaphase chromosomes are not stained by LAP2 antibodies, and that anaphase chromosomes are stained only in regions where the nuclear envelope has begun to reassemble. Bars, 10 µm. (C) LAP2 isoforms of zebrafish AB9 cells. Total proteins of AB9 cells were separated by SDS-PAGE (11% acrylamide) and immunoblotted with ZLAP2-serum1. ZLAP2 is the major isoform in AB9 cells, and only minor amounts of ZLAP2ß were detectable. Identical results were obtained with zebrafish ZF4 cells (data not shown). Molecular masses of reference proteins (in kDa) are marked.