Fig. 8. Intracellular distribution of ZLAP2-GFP fusion proteins in transfected Xenopus A6 cells during interphase and cell division. In all constructs GFP was fused to the C-terminus of full-length LAP2 isoforms. Transfected cells expressing ZLAP2- GFP (A,B), ZLAP2ß-GFP (C) and ZLAP2-GFP (D) are shown during interphase (A) and mitosis (B-D). The GFP fluorescence (A-D) and the same cells after staining with lamin B2 antibodies (A') or the DNA dye Hoechst (B'-D') are shown. ZLAP2-GFP is distributed in mitotic cells throughout the cytoplasm and forms local aggregates (D), whereas ZLAP2- GFP (B) and ZLAP2ß-GFP (C) predominantly colocalized with chromatin. Digital images were taken with a Leica CLSM (A,A') and a Zeiss Axiophot (B-D'). Bars, 10 µm.