Fig. 9. Intracellular distribution of deletion mutants of ZLAP2 expressed as GFP fusion proteins in transfected Xenopus A6 cells during cell division. In all constructs, GFP was fused to the carboxyterminus of LAP2 deletion mutants. Transfected cells expressing mutant 1-214GFP (A; amino acids 1-214 common to all ZLAP2 isoforms), mutant 1-360GFP (B, amino acids 1-360 of ZLAP2ß) and mutant 214-502GFP (C; amino acids 214-502 of ZLAP2) are shown during mitosis. The GFP fluorescence (A-C) and the same cells after staining with the DNA dye Hoechst (A'-C') are shown. Note the association of mutant 1-360GFP with mitotic chromosomes and the exclusion of 214-502GFP from regions containing chromosomes. Digital images were taken with a Leica CLSM (A-C) and a Zeiss Axiophot (A'-C'). Bars, 10 µm.