Fig. 5. Mutation of both potential PtdIns(4,5)P₂-binding motifs altered subcellular localization of FERM domain as well as full-length PTPL1 in HeLa cells. HA-epitope tagged (A-C) PTPL1-FERM domain or (D-F) full-length PTPL1 carrying KN1-2 mutations were transiently expressed in HeLa cells. Cells were treated for indirect immunofluorescence of (A,D) PTPL1 constructs and (B,E) F-actin as described in Fig. 2. (C,F) Dual localizations are merged in color. Images represent merges of horizontal confocal planes. (A,E) Both constructs displayed a similar cytosolic localization enriched around nucleus (arrows). Bars, 20 µm.