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Fig. 3. Co-immunoprecipitation of different epitope-tagged wild-type and truncated Xfz3 receptors. Embryos were injected with the mRNA encoding the indicated receptor (Xfz3-myc or Xfz3{Delta}C-myc) alone or in combination with Xfz3-HA. Protein extracts from injected embryos were subjected to immunoprecipitation by using the anti-HA antibody (lanes 1, 3, 5, 7) or the anti-myc antibody (lanes 2, 4, 6, 8). Immunoprecipitates were then immunoblotted with the anti-myc antibody. Anti-HA antibody does not immunoprecipitate any myc-positive bands from embryos expressing only Xfz3-HA (lane 7) or Xfz3{Delta}C-myc (lane 1) but does precipitate bands corresponding to Xfz3{Delta}C-myc in co-injected embryos (lane 3). Similar results are obtained when Xfz3{Delta}C-myc is replaced by the full-length Xfz3-myc (lane 5).