Fig. 4. Xfz3 dimerization is sensitive to ß-mercaptoethanol. Protein extracts
from embryos overexpressing Xfz3
C-myc were analyzed by western blot
with the anti-myc antibody. Before SDS-PAGE, protein samples were incubated
with 2% or 20% ß-mercaptoethanol. This treatment leads to a diminution of
the dimer/monomer ratio. The dimeric form of the receptor is still strongly
detected in extracts treated with 10 mM iodoacetamide (IAA), which prevents
the artificial formation of disulfide bonds during sample preparation.