Fig. 1. Sla1118-511p is expressed in cells and localises to the cell cortex. (A) Schematic diagram of Sla1p and Sla2p showing domain structure and regions of Sla1p thought to be important for its role in regulating the actin cytoskeleton. (B) Cells expressing full-length Sla1p, Sla1(118-511)p or cells in which sla1 was deleted (KAY302, 351 or 97 respectively) were grown overnight and cell extracts made. Proteins were separated by SDS-PAGE and western blotted using antibodies against Sla1p. As shown, proteins of the appropriate size were found in cells expressing Sla1p. No band was observed in the extract from sla1 cells. As a loading control the same blots were probed with antibodies to a nuclear protein Anc1p. This showed equivalent levels in all lanes. (C) Log phase KAY303 and KAY367 cells expressing Sla1-myc and Sla1(118-511)myc were grown to log phase and processed for immunofluorescence microscopy. Both Sla1p-myc and Sla1(118-511)-myc localise to punctate patches at the cell cortex demonstrating that the parts of Sla1p required for cortical localisation are located elsewhere in the protein. Bar, 5 µM.