Fig. 2. Sla1p interacts with Sla2p in a two-hybrid screen. A two-hybrid screen was performed as described in Materials and Methods with a bait plasmid carrying the amino acid 118-511 region of Sla1p. The minimum interacting sequence was between residues 310 and 768 of Sla2p. Two-hybrid interactions between a number of different Sla1 and Sla2 constructs were tested, as shown. (A) Summary of domain interactions. The strength of interaction was determined by the extent of growth after 5 days with +++ denoting the greatest growth. Corresponding to positions on this table are strains which in B are growing on synthetic medium lacking uracil and leucine, indicating that both plasmids are present, and in C are growing on synthetic medium lacking histidine, adenine, uracil and leucine, indicating that a positive interaction between Sla1p and Sla2p has taken place.