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Fig. 4. Sucrose density gradient fractionation of wild-type and {Delta}sla1 cells. Extracts from KAY302 (wildtype) and KAY300 ({Delta}sla1) cells were prepared and separated on 3-30% sucrose gradients as described in Materials and Methods. Fractions were then run on SDS-PAGE and blotted onto PVDF membranes. The membrane was cut and probed with individual antibodies. Antibodies were used as described in Materials and Methods. Quantitation of band intensity was performed using NIH Image 1.6.1 Software. The y-axis of the graphs is the band intensity in arbitrary units normalised in each case for background. The gradients were repeated three times. Shown here is a representative example.