Fig. 7. Rvs167p localises with actin and not Sla2p in sla1 cells. KAY302 (wild-type) and KAY 97 (sla1) cells were processed for immunofluorescence microscopy as described in Materials and Methods. Actin was stained using rhodamine-phalloidin, and Rvs167p was localised using antibodies raised against the protein. Although actin organisation is altered in the sla1 strain, Rvs167p still localises to these aberrant structures, which suggests that its association with actin is not mediated by Sla1p. Bar, 10 µM.