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Fig. 9. Endocytosis and membrane trafficking in {Delta}sla1{Delta}sla2 cells. (A) Fluid phase endocytosis was monitored by following uptake of the fluorescent dye lucifer yellow (see Materials and Methods). Wild-type cells show uptake of the dye into vacuoles. Over the same time period {Delta}sla1 cells show a reduction in uptake whereas {Delta}sla2 and the {Delta}sla1{Delta}sla2 cells show little or no detectable internalisation of the dye. Bar, 10 µm. The percentage of cells showing detectable uptake of the dye into vacuoles is summarised graphically in B. 200 cells were counted in each of three experiments. Error bars represent standard error. (C) Wild-type (D) {Delta}sla1, (E) {Delta}sla2 and (F) {Delta}sla1{Delta}sla2 cells were processed for electron microscopy using potassium permanganate as described in Materials and Methods. Bar, 5 µm.