Fig. 5. I-A^k-mediated signaling in SaI/A^k cells induces recruitment of PKC- into DIGs. (A) Soluble and DIG fractions from SaI/A^k and SaI/A^k tr cells, stimulated or not via I-A^k for 15 minutes, were immunoblotted with anti-PKC-, -, -µ Abs. Only the PKC- isoenzyme was detected in DIGs isolated from either SaI/A^k or SaI/A^k tr. (B) Cholesterol dependence of PKC- recruitment was analyzed in SaI/A^k cells pretreated or not with MßCD for 15 minutes and stimulated or not via I-A^k for 15 minutes. Soluble and DIG fractions were immunoblotted (IB) with anti-PKC- Ab. Pretreatment of SaI/A^k with MßCD completely abrogated PKC- recruitment to DIGs.