Fig. 1. Localisation of IRSp53 in B16 melanoma cells. (a-d) Cells transfected with myc-IRSp53 were cultured on laminin and immunolabelled with monoclonal anti-myc antibody 9E10 (a,c) and rhodamine-phalloidin (b,d). Arrows in (a,b) indicate the lamellipodium extending from the cell moderately expressing IRSp53. Arrowheads in (a,b) indicate an overexpressing cell with branched filopodial extensions. Double-arrowheads point to the non-lamellipodium cell membrane. Bars, 10 µm.