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Fig. 3. Localisation of myc-tagged IRSp53 constructs in B16 cells. (a) Myc-tagged IRSp53 constructs. RCB, CRIB and SH3 indicate Rac binding region, CRIB motif and SH3 domain, respectively. Number indicates the position of corresponding amino acid residue. (b-i) Cells transfected with myc-{Delta}SH3, myc-SH3 or myc-RCB, as indicated, and immunolabelled with monoclonal anti-myc antibody 9E10 (b,d,f,h) and rhodamine-phalloidin (c,e,g,i). Arrows and arrowheads indicate the tips of filopodia and lamellipodia, respectively. Bars, 10 µm.