Fig. 5. Localisation of WAVE2 in B16 cells plated on laminin. (a,b) A cell immunolabelled with an anti-WAVE antibody that recognises all three WAVE isoforms (a) and with rhodamine-phalloidin (b). (c,d) Sequential video frames taken in fluorescence (c) and phase contrast (d) of a cell that was transfected with WAVE2- DsRed. For living cell observation, lamellipodia protrusion was stimulated by treatment with aluminium fluoride 15 minutes before image acquisition. White line indicates region of retraction followed by lamellipodium protrusion; arrows and arrowheads indicate filopodia. Time is in seconds. Bars, 10 µm.