Fig. 1. Src^527F/c-Yes chimeric constructs. Src^527F/c-Yes chimeras are depicted subdivided into the following functional domains: SH4 domain; Unique domain; SH3 domain; SH2 domain; protein tyrosine kinase (PTK) domain; and regulatory sequence (Reg). Gray boxes represent domains from c-Yes; white boxes represent domains from Src^527F. The generation of the Y3^527F, Y2^527F, Y32^527F, Y4U32^527F, Y4U^527F, Y4^527F and YU^527F chimeric constructs has been described previously (Summy et al., 2000). All proteins were expressed in CEF via the Rous Sarcoma Virus (RSV) vector.