Fig. 3. Expression of Src^527F/c-Yes N-terminal chimeras in CEF and their effects on cell morphology and the actin cytoskeleton. (A) The SH4-Unique domains of c-Yes prevent Src^527F from affecting cell morphology. Confluent CEF that were uninfected, or expressing Y4U32^527F, Y32^527F Y4U^527F, Y4^527F or YU^527F were photographed at day 12 post-transfection in 100 mm tissue culture plates (Falcon). Cells were photographed at 40x total magnification using a Plan 2 filter. (B) The SH4-Unique domains of c-Yes prevent Src^527F from affecting actin filament integrity. CEF expressing Y4U32^527F, Y32^527F Y4U^527F, Y4^527F or YU^527F were fixed at 50% confluence on coverslips in 3.7% formaldehyde, permeablized in 0.4% Triton X-100, and stained with rhodamine-phalloidin (2 µg/ml). Cells were visualized via Zeiss LSM 510 confocal microscopy (63x objective). Bars, 10 µm.