Fig. 4. Centrin deficiency caused mistakes during bb segregation. (A) Histograms showing the distribution of flagella (solid bars) and centrin dots (open bars) in control and A10 cells. Note the discrepancy between the numbers of centrin dots (presumably bbs) and that of flagella in the centrin-RNAi strain. (B) Analysis of mitotic and dividing cells from control (a-c), F11 (d-g), A10 (h-k) and N41 (l,m). Detergent-extracted cells (a-j) or methanol-permeabilzed cells (k-m) were stained with anti--tubulin (not in l,m) and anti-centrin (BAS6.8); cell nuclei were stained with DAPI. While centrin and the bbs were located at the spindle poles of wild-type cells (arrowheads in a), bbs were detached from the poles in centrin-deficient cells. Frequently, mistakes in bb segregation were observed in mitotic or dividing cells (arrowhead in g,i-m). Arrows, metaphase band microtubules; arrowhead in j, spindle without bbs; arrowheads in m, dividing cells; l, phase contrast. Bars, 5 µm (in j for a-j).