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Fig. 7. Centrin deficiency increased the number of multinucleated cells. (a) N41 cell with three pyrenoids (arrowheads). (b) Phase contrast of a detergent-permeabilized N41 cell stained with Syto25 (Molecular Probes, Leiden, NL) revealing three nuclei (c). Bars, 5 µm (a), 10 µm (b,c). (d) Histogram showing the frequency of multinucleated cells in control, A10 and N41.