Fig. 4. Analysis of anti- and pro-apoptotic proteins under hypoxic conditions. Cells collected after 24 hours of culture under normoxic or hypoxic conditions were subjected to immunostaining using specific antibodies and FACS analysis. The ratio of Bcl-2 to Bax dropped from 1.36 in normoxia to 0.79 in hypoxia. Data were expressed as means±s.e.m. pooled from duplicate samples of three experiments (*P<0.05 and **P<0.01, student's t-test).