Fig. 3. Heat shock induces the cytosolic redistribution of IL-1 and S100A13. NIH 3T3 cells were transiently cotransfected with IL-1-DsRed1 and EGFP-S100A13 constructs. 24 hours following transfection, the cells were fixed after a 2 hour incubation at either 37°C (a-c) or 42°C (d-f). Confocal images of median horizontal cell sections were obtained and the intracellular distribution of IL-1-DsRed1 (a,d), EGFP-S100A13 (b,e) and their respective overlays (c,f) recorded.