JCS -- Mandinova et al. 116 (13): 2687 Figure IG5

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Fig. 5. IL-1 ${alpha}$ exhibits the ability to displace carboxyfluorescein from the dioleylphosphoglycerol micelles. DOPG liposomes containing CF were prepared as described in Materials and Methods; the micelle was introduced into the cuvette at a 1:60 dilution in 10 mM HEPES, pH 7.0 containing 150 mM NaCl and allowed to equilibrate. At the 120 second time point, the experiment was initiated by introducing either 0.1% Triton X-100 (positive control, ${bullet}$ ), 0.5 µM of ${alpha}$ -Chymotrypsin (negative control, —), and different concentrations of IL-1 ${alpha}$ , 0.5 µM ( ${circ}$ ), 0.25 µM ( ${blacktriangleup}$ ), 0.125 µM ( ${square}$ ) 0.062 µM (x) and 0.031 µM ( ${diamond}$ ).