Fig. 1. Acceleration of EDEN-dependent deadenylation in calcium-activated CSF extract. (A) Capped, radiolabelled transcript Eg2-410 and Eg2-410a were incubated in CSF- or calcium (0.8 mM)-activated extracts for 1, 2 or 3 hours. After extraction and precipitation RNAs were analysed on urea/acrylamide 4% gels. The positions of the different forms of the transcripts are indicated: (A⁺), fully adenylated (A65) form, and (A^-), fully deadenylated form. The lane (T) corresponds to the fully adenylated radiolabelled transcripts without previous incubation with extract and precipitation. (B) Sperm nuclei chromatin was stained with Hoechst dye and observed in fluorescence and phase contrast. Condensed sperm chromosomes in the CSF extract (left) and decondensed nuclei in calcium activated extract (right).