Fig. 5. Roscovitine downregulates EDEN-dependent deadenylation. Roscovitine was added (+) or not (-) to calcium-activated CSF extract to a final concentration of 100 µM. Capped, radiolabelled transcript Eg2-410 and Eg2-410a were incubated in the extract for 1, 2 or 3 hours at 21°C. After extraction and precipitation radiolabelled RNAs were analysed on urea/acrylamide 4% gel. The lane (T) corresponds to the fully adenylated radiolabelled transcripts without previous incubation with extract and precipitation.