Fig. 6. Okadaic acid downregulates EDEN-dependent deadenylation and inhibits dephosphorylation of EDEN-BP. (A) Okadaic acid (OA) at 1 µM final concentration was added (+) or not (-) to CSF extract and incubated for 15 minutes at 21°C. Both extracts were then supplemented with CaCl₂ (to final concentration of 0.8 mM) and incubated at 21°C for 1, 2 or 3 hours with radiolabelled transcripts Eg2-410 and Eg2-410a. RNA were extracted, analysed on urea/polyacrylamide 4% gel and autoradiography. The lane (T) corresponds to the fully adenylated radiolabelled transcripts without previous incubation with extract and precipitation. (B) Proteins were resolved on 15% Anderson SDS/PAGE, transferred to membrane and immunoblotted with anti-EDEN-BP antibody. The different electrophoretic forms of EDEN-BP are indicated both on the left and on the right.