Fig. 1. BFA effects the organisation of living pollen tube tips. Confocal images of FM4-64 distribution in L. longiflorum (A,B) and N. plumbaginifolia (C,D) before and 30-60 minutes after 3.6 µM BFA treatment. (E-F) Confocal images of mitofluor staining before and after BFA treatment in L. longiflorum. (G-H) Schematic of cytoplasmic movements in (G) growing and (H) BFA-treated pollen tube, based upon image sequences. (I) Projected time series of an FM4-64-stained, BFA-treated tube showing tracks of movement. Bars, 15 µm (A,B,E,F) or 10 µm (C,D); corresponding bright field images shown at one-third size.