Fig. 5. ER and Golgi localisation in growing L. longiflorum pollen tubes shown by targeted fluorescent protein expression. (A-B) mGFP5-ER in (A) growing tube, sequential images (image/3 seconds) showing the dynamic nature of ER; (B) onion epidermis, showing the typical reticulate ER structure. (C-E') GT-EYFP: (C) Sequential images (image/2 seconds) of a growing tube revealing movement of targeted structures; (D) high expression levels; (E) in onion epidermis; (E') effects of BFA on labeling in onion epidermis. (F-G) GONST1-YFP expressed in growing L. longiflorum pollen tubes: (F) Sequential images (as for C); (G) high expression levels showing labelling of the apical vesicle accumulation. Inserts show bright field images at one-third size. Bars, 15 µm (A,C,D,F,G); 100 µm (B,E).