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Fig. 3. Meu14 is required for the proper segregation of SPBs. (A) Chromosomal DNA, SPBs, microtubules in meu14{Delta} (YDO100) cells were stained by Hoechst 33342 (blue), anti-Sad1 antibody (green) and anti-{alpha}-tubulin antibody TAT-1 (red), respectively. Merged images are also shown. The following typical images are displayed. (i) Microtubule extension between the two pairs of dividing nuclei at meiosis II is not synchronized. (ii) Multiple foci of Sad1 staining are observed. (iii) Microtubules between the two nuclei are not detected. (iv) Localization of microtubules is abnormal and there are more than four Sad1 foci. (v,vi) meu14{Delta} (YDO100) cells at metaphase I (v) or anaphase I (vi). (B) Sad1 colocalizes with the SPB protein Spo15 during meiosis II. The following typical images are displayed for meu14{Delta} (YDO111) (i-iii) or wild-type (iv,v) cells. (i) One of the four SPBs is abnormal. (ii) More than four SPBs are observed. (iii) Nuclei are not properly divided at meiosis II. (iv,v) Wild-type cells at metaphase II (iv) and anaphase II (v). Bar, 10 µm.