Fig. 1. Human mitofusin homologues Mfn1 and Mfn2 aligned with Drosophila Fzo (ClustalW and Boxshade). Black shading: amino acid identity. Gray shading: amino acid similarity. Asterisks: G1, G2, G3, G4 (four motifs of the signature GTPase domain). Thick brackets: region used to generate Mfn1-specific antibodies. Parentheses: internal peptide sequence used as imunogen for Mfn2-specific antibodies.