Fig. 2. Effect of moPrnp-specific siRNA on the accumulation of PrP-res in GT-1 cells infected with 22L mouse scrapie strain. Infected GT-1 cells were transfected in the absence (0) or in the presence of specific or scrambled siRNA duplexes. Four days post-transfection, cells were lysed and the post-nuclear supernatants were PK-treated as described in Materials and Methods. PrP-res was detected with the SAF83 monoclonal antibody. The blot was developed by using an enhanced chemiluminescence system and exposed on x-ray film. Brackets on the right side indicate the immuno-detected bands corresponding to the un-, mono- and diglycoforms of PrP-res. Molecular mass markers in kilodaltons (kDa) are indicated on the left.