Fig. 4. Localization of Rec8 protein on synaptonemal complexes during meiotic prophase. Nuclear spreads from testicular cells were prepared as described in Materials and Methods and subjected to immunofluorescent double labeling with anti-Rec8 and anti-SCP3 antibodies. (A) Leptotene; (B) zygotene; (C) pachytene; (D) diplotene. An arrow indicates an unsynapsed XY bivalent. Bar, 10 µm.