Fig. 6. Localization of Rec8 protein on chromosomes in meiosis I and II. Meiotic chromosome spreads were prepared to prevent chromatin dispersion by SDS during fixation. The chromosome spreads were immunofluorescently stained with anti-Rec8 antibody, and DNA was counter-stained with propidium iodide. (A) Pachytene; (B) diakinesis; (C) metaphase I; (D) early anaphase I; (E) late anaphase I; (F) metaphase II; (G) anaphase II; (H) mitosis. The regions indicated in C and D are magnified in I and J, respectively. Arrows indicate Rec8 signals on the interstices between sister chromatids. White arrowheads indicate Rec8 signals on the overlapped sites of two homologous chromosomes. Blue arrowheads indicate Rec8 signals on the interstitial axes between homologous chromosomes (chromosome arm regions distal to chiasmata). Bar, 5 µm.