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Fig. 6. Changes in InsP3R-3 and ZO-1 localization in MDCK cells during calcium switch. Polarized MDCK cells were incubated for 2 hours in medium deprived of calcium and then incubated in a calcium-containing medium for 24 hours. (A) TER measurement during this treatment; the arrow indicates the time when the cells were renewed with calcium-containing medium. (B) InsP3R-3 and ZO-1 localization. Bar, 10 µm.