Fig. 6. Effects of Myc-Lin-2/Pals1 PDZ chimera expression on junctional assembly
and apico-basal polarity in MDCK monolayers. (A-B) MDCK stable cell lines
expressing either Myc-Lin-2/Pals1 PDZ or Myc-Lin-2
PDZ (negative
control) were subjected to a calcium switch experiment as in
Fig. 3. At the indicated
timepoints, cells were co-immunostained with anti-ZO-1 and anti-E-cadherin
antibodies (green and red, respectively). (C) MDCK cells expressing the
indicated proteins were grown in low calcium media (t=0) or in normal calcium
media for 24 hours. Subsequently, they were co-stained with antibodies
directed against E-cadherin (red) and either gp135 or CRB3 as indicated in
green.