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Fig. 7. Effects of Myc-Lin-2/Pals1 PDZ chimera expression on apico-basal polarity in MDCK cysts cultured in collagen gels. (A) Cysts derived from MDCK cells expressing Myc-Lin-2{Delta}PDZ were grown in collagen matrix and co-stained with either anti-Myc/rhodamine-phalloidin or anti-E-cadherin/anti-gp135 antibodies. (B) In parallel, cysts derived from MDCK cells expressing the Myc-Lin-2/Pals1 PDZ chimeric protein were co-immunostained with the indicated antibodies.