Fig. 2. Dd-STATa shows neither osmotic stress or 8-bromo-cGMP-induced nuclear translocation. Ax2 cells were allowed to develop for 4 hours in shaken suspension and divided into five. One portion was incubated in KK2 alone, and the other portions were incubated in KK2 with the indicated additions (5 mM cAMP, 200 mM sorbitol, 100 nM DIF or 20 mM 8-bromo cGMP). After 3 minutes of incubation, two aliquots were removed. In one aliquot cells were analysed immunohistochemically to determine the proportion of cells showing nuclear enrichment of Dd-STATa (dark grey boxes), and in the other aliquot the proportion of cells showing nuclear enrichment of Dd-STATc was measured (light grey boxes). The results are shown as the mean±s.d.